Plasmids
Plasmid name Backbone plasmid Insertion Bacterial Marker Yeast Marker Reporter Length Yeast Origin Bacterial Origin Map file Notes creator

pJD741 FLR1













































pJD427 ppr1 whole









pJD741 FKS1









pJD741 (pJD672)NUP82 1494-1707 + pJD375









pJD741 (pJD667)EST2 1956-2142 + pJD375









pJD741 ppr1.525 (pJD746)









pRS315 ppr1 whole










est2 whole









pJD741 (pJD659)EST2 12-183 + pJD375









pJD427 est2 whole









pJD0429 RPL10
ura3
6729

Genbank_Plasmid_Maps/pJD429.gb RPL10 in pRS426 from A Johnson lab, corrected by A. Petrov




Ura3



Genbank_Plasmid_Maps/pRS426.gb

2micron_doublet







Genbank_Plasmid_Maps/2micron_doublet.gb

est2 pJD741 (pJD661)EST2 1284-1473 + pJD375








p2luc

AmpR

5405

Genbank_Plasmid_Maps/p2luc.gb

p362
LA frameshift signal






1st LA expression vector, from Reed Wickener
p375 pBR322 Entire LA cDNA






Entire LA cDNA, also inserted TRP1, 2u origin, PGK1 promoter
pBluescript SK+

AmpR
LacZ 2958

Genbank_Plasmid_Maps/pBluescriptSK+.gb

pBluescriptIISK-

AmpR

2961

Genbank_Plasmid_Maps/pBluescriptSKII-.gb

pBluescriptSK-



LacZ 2958

Genbank_Plasmid_Maps/pBluescriptSK-.gb

pBluescriptSKII-







Genbank_Plasmid_Maps/pBluescriptSKII-.gb

pBR322

AmpR

4361

Genbank_Plasmid_Maps/pBR322.gb This is the parent of pretty much everything.
pCM182


TRP1
7407

Genbank_Plasmid_Maps/pCM182.gb

pCM184







Genbank_Plasmid_Maps/pCM184.gb

pDJ0425 pCM185

trp1
7730

Genbank_Plasmid_Maps/pJD425.gb This is pCM185
pF8 pT1 LA






pT1 with LA, additional Gs, deletions for frame maintenance
pF'8 pTI24 M13 OoR






This is the parent of most/all frameshift vectors in the lab. Added M13 origin of replication into unique SalI site of pF8(or pTI24, unclear)
pJD0034 p375 LA mutants








pJD0038 p375 LA mutants








pJD0039 p375 LA mutants








pJD0040 p375 LA mutants








pJD0041 p375 LA mutants








pJD0042 p375 LA mutants








pJD0045 pF'8 LA mutants








pJD0046 pF'8 LA mutants








pJD0047 pF'8 LA mutants








pJD0048 pF'8 LA mutants








pJD0049 pF'8 LA mutants








pJD0050 pF'8 LA mutants








pJD0051 pF'8 LA mutants








pJD0052 pF'8 LA mutants








pJD0053 pF'8 LA mutants








pJD0054 YEP13 suf1






822bp fragment with tRNA gene and SalI/HindIII sites.
pJD0055 pF'8/p362 Ty1 and LA in PRF








pJD0056 pF'8/p362 Ty1 and LA full cDNA (BIG)








pJD0057 pF'8/p362









pJD0058 pF'8/p362









pJD0059 pF'8/p362









pJD0060 pF'8/p362









pJD0068 pF'8 Ty1








pJD0069 pF'8 Ty1








pJD0070 pF'8 Ty1








pJD0071 p362 Ty1








pJD0072 p362 Ty1








pJD0073 p362 Ty1








pJD0077 pBluescript SK- LA PRF signal






Used to generate T7 transcripts of LA frameshift signal
pJD0084
ClaI from pRS305








pJD0085 pRS308/pF'8 4.9kb HindIII from pF'8








pJD0085 pJD0085/pTI25









pJD0120.0







Genbank_Plasmid_Maps/pJD120.0.gbk

pJD0120-XbaI p0luc / pJD120 /pBluescript
AmpR

4556

Genbank_Plasmid_Maps/pJD120-XbaI.gbk one XbaI site has been inactivated
pJD0146 pBluescript II SK triple Hatag AmpR none lacZ >2961
f1/


pJD0147 pRS304 tcm full length gene Amp Trp Lacz >4271 ori/Cen6 ori/Cen6
tcm1 is in the reverse orientation
pJD0148 pRS304 tcm from Peltz in BamHI cassette Amp Trp Lacz >4271 ori/Cen6 ori/Cen6


pJD0149 pBluescript II SK triple Hatag AmpR none lacZ >2961
f1/


pJD0150 pRS304 PGK1p and lacZ into multicloning site AmpR TRP1 lacZ >4271 ori/CEN6 ori/CEN6


pJD0157 pGEM CYH2






CYH2 inserted with EcoRI and AccI, equivalent to Peltz's p3433
pJD0175a pJD175e MMTV AmpR

5450

pJD175a.gb

pJD0175b pJD175e MMTV control AmpR




pJD175b.gb

pJD0175c pJD175e HIV AmpR




pJD175c.gb

pJD0175d pJD175e HIV control AmpR




pJD175d.gb

pJD0175e
None AmpR
dual lux

ColE1, no rop pJD175e.gb

pJD0175f
None AmpR
dual lux

ColE1, no rop pJD175f.gb

pJD0187.wt
dual luciferase AmpR

5447

pJD187.wt.gb

pJD0191 p3082 PGK1 with UGA at the KpnI site AmpR




pJD191.wt.gb PGK1 with UGA at the kpnI site
pJD0192 p3131 wild type PGK1 AmpR




pJD192.wt.gb wild type PGK1
pJD0194







pJD194.wt.gb Pinger plasmids
pJD0195







pJD195.wt.gb Pinger plasmids
pJD0196







Genbank_Plasmid_Maps/pJD187.wt.gb Pinger plasmids
pJD0216







Genbank_Plasmid_Maps/pJD187.wt.gb Pinger plasmids
pJD0217







Genbank_Plasmid_Maps/pJD187.wt.gb Pinger plasmids
pJD0255







Genbank_Plasmid_Maps/pJD187.wt.gb Pinger plasmids
pJD0256







Genbank_Plasmid_Maps/pJD187.wt.gb Pinger plasmids
pJD0257







Genbank_Plasmid_Maps/pJD187.wt.gb Pinger plasmids
pJD0258







Genbank_Plasmid_Maps/pJD187.wt.gb Pinger plasmids
pJD0259







Genbank_Plasmid_Maps/pJD187.wt.gb Pinger plasmids
pJD0269







Genbank_Plasmid_Maps/pJD187.wt.gb Pinger plasmids
pJD0270







Genbank_Plasmid_Maps/pJD187.wt.gb Pinger plasmids
pJD0273







Genbank_Plasmid_Maps/pJD187.wt.gb Pinger plasmids
pJD0274







Genbank_Plasmid_Maps/pJD187.wt.gb Pinger plasmids
pJD0277







Genbank_Plasmid_Maps/pJD187.wt.gb Pinger plasmids
pJD0278







Genbank_Plasmid_Maps/pJD187.wt.gb Pinger plasmids
pJD0305 pRS316 new AatII and NheI sites AmpR Ura3 PGK1


pJD305.gb pW9, Old Kpn1 site destroyed, reading frame maintained, can clone frameshift signals in as AatII/NheI or XbaI fragments
pJD0306 p3131 AatII and NheI sites AmpR Ura3 PGK1


pJD306.gb p3131(Peltz), Can clone frameshift signals as AatII/NheI (XbaI) fragments
pJD0307 pTD160.0/kpnI ABP-F (203bp)

+1 PRF


Genbank_Plasmid_Maps/pJD187.wt.gb ABP sequence for assay +1 frameshifting
pJD0308 pTD160.0/kpn1 ABPn / kpnI





Genbank_Plasmid_Maps/pJD187.wt.gb ABPm (100) / kpnI, insert sequence is same as in pJD277
pJD0309 p3131 / pJD305 LA-fragment





pJD309.gb Kpn1 site of pRS316 removed, BamHI/HindIII from p3131 into pW9(pJD305), LA fragment digested with Kpn1 cloned into kpn1 site of PGK1
pJD0310 p3131 / pJD305 LA-fragment AmpR Ura3 PGK1


pJD310.gb Kpn1 site of pRS316 removed, BamHI/HindIII from p3131 into pW9(pJD305), LA fragment digested with Kpn1 cloned into kpn1 site of PGK1
pJD0311 pJD210 / pRS426 NotI/SalI site from pJD210 (35S rRNA) AmpR Ura3
5726 2u ColE1 pJD311.gb 35S rRNA insertion using NotI/SalI fragment from pJD210
pJD0312 pNT22A PAPx





Genbank_Plasmid_Maps/pJD187.wt.gb Plasmids from Nilgun Tumer "pNT22A in NT198, PAPx"
pJD0313 pNT246 PAPc





Genbank_Plasmid_Maps/pJD187.wt.gb Plasmids from Nilgun Tumer "pNT246 in NT198, PAPc"
pJD0314 pNT255 PAPn





Genbank_Plasmid_Maps/pJD187.wt.gb Plasmids from Nilgun Tumer "pNT255 in NT198, PAPn"
pJD0315 pBluescript SK X





pJD315.gb Rosa Esteban, "X cloned into pBluescript SK SxxxI site", Site-specific binding of viral pls single-stranded RNA to replicase-containing open virus-like particles of yeast
pJD0316 pSAL1, pRS315






Genbank_Plasmid_Maps/pJD187.wt.gb Leu2 and Ura3 separated by "IGR IRES" -- Peter Sarnow's lab
pJD0317
CrPV IRES





Genbank_Plasmid_Maps/pJD187.wt.gb "CrPV IRES cloned into ?" CrPV1-1 from Peter Sarnow's lab
pJD0318 pRIP1

Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRIP UPF1, mutant domain: NA
pJD0319 pRS426

Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRIP UPF1, mutant domain: NA
pJD0320 pRIP1 C65S
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRIP UPF1, pRIP1, mutant domain: cysteine/histidine rich
pJD0321 pRS426 C65S
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRS UPF1, pRS426, mutant domain: cysteine/histidine rich
pJD0322 pRIP1 c72S
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRIP C65s, pRIP1, mutant domain: cysteine/histidine rich
pJD0323 pRIP1 c84s
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRS C65s, pRS426, mutant domain: cysteine/histidine rich
pJD0324 pRS426 h94r
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRIP C72s, pRIP1, mutant domain: cysteine/histidine rich
pJD0325 pRIP1 h94r
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRIP C84s, pRIP1, mutant domain: cysteine/histidine rich
pJD0326 pRS426 h94r
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRS H94R, mutant domain: cysteine/histidine rich
pJD0327 pRIP1 h98r
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRIP H98R, mutant domain: cysteine/histidine rich
pJD0328 pRS426 h98r
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRS H98R, mutant domain: cysteine/histidine rich
pJD0329 pRIP1 h110r
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRIP H110R, mutant domain: cysteine/histidine rich
pJD0330 pRS426 h110r
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRS H110R, mutant domain: cysteine/histidine rich
pJD0331 pRIP1 c122s
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRIP C122S, mutant domain: cysteine/histidine rich
pJD0332 pRS426 c122s
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRS C122S, mutant domain: cysteine/histidine rich
pJD0333 pRIP1 c125s
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRIP C125S, mutant domain: cysteine/histidine rich
pJD0334 pRS426 c125s
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRS C125S, mutant domain: cysteine/histidine rich
pJD0335 pRIP1 c148s
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRIP C148S, mutant domain: cysteine/histidine rich
pJD0336 pRS426 c148s
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRS C148S, mutant domain: cysteine/histidine rich
pJD0337 pRIP1 de572aa
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRIP DE572AA, mutant domain: helicase
pJD0338 pRS426 de572aa
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRS DE572AA, mutant domain: helicase
pJD0339 pRIP1 rr793aa
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRIP RR793AA, mutant domain: helicase
pJD0340 pRS426 rr793aa
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRS RR793AA, mutant domain: helicase
pJD0341 pRIP1 tr800aa
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRIP TR800AA, mutant domain: helicase
pJD0342 pRS426 tr800aa
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pRS TR800AA, mutant domain: helicase
pJD0343
pmof4-1 (c62y)
Ura3



Genbank_Plasmid_Maps/pJD187.wt.gb pmof4-1 (C62Y), mutant domain: cysteine/histidine rich
pJD0344 pYES2/NT A
AmpR Ura3
6038

Genbank_Plasmid_Maps/pJD344.gb I think this came directly from invitrogen
pJD0345 pYES2/NT B
AmpR




Genbank_Plasmid_Maps/pJD345.gb

pJD0346 pYES2/NT C
AmpR Ura3
6038

Genbank_Plasmid_Maps/pJD346.gb

pJD0347 pYES2/NT/lacZ lacZ ORF AmpR Ura3
9101 2u
Genbank_Plasmid_Maps/pJD347.gb

pJD0348 pJR63 WT L-A cDNA clone
Ura3



Genbank_Plasmid_Maps/pJD347.gb pJD348 is pRS63, WT L-A cDNA clone, URA3 marker (R. Wickner)
pJD0349 p614 L-A clone variant
TRP1

2u
Genbank_Plasmid_Maps/pJD347.gb pJD349 is p614, L-A clone variant, pTIL131 S178P = wt, TRP1, 2u (Reed Wickner)
pJD0350 pl2L2






Genbank_Plasmid_Maps/pJD347.gb "pJD250 is pl2L2 J Harger 5/20/02, I will put map in soon"
pJD0351 YCPLac33






Genbank_Plasmid_Maps/pJD347.gb "pJD351 is pL3Delta-100 in YCPLac33"
pJD0352 p616 L-A clone variant
TRP1

2u
Genbank_Plasmid_Maps/pJD347.gb pJD352 is p61 (Wickner), pTIL131 S178P H154N, L-A clone variant
pJD0353 pRS314 none





Genbank_Plasmid_Maps/pJD347.gb I think this is pRS314
pJD0354 pRS314 wt control, targets G2811





Genbank_Plasmid_Maps/pJD347.gb pBL152 (wt control, targets methylation to G2811)
pJD0355 pRS314 targets U2950





Genbank_Plasmid_Maps/pJD347.gb pBL232 (targets methylation to U2950)
pJD0356 pRS314 targets A2967





Genbank_Plasmid_Maps/pJD347.gb pBL233 (targets methylation to A2967)
pJD0357 pRS314 targets C2817





Genbank_Plasmid_Maps/pJD347.gb pBL226 (targets methylation to C2817)
pJD0358 p3082


PGK1


Genbank_Plasmid_Maps/pJD347.gb Peltz plasmid p3082 (Peltz et al 1993, Genes and Development)
pJD0359 p3131


PGK1


Genbank_Plasmid_Maps/pJD347.gb Peltz plasmid p3131 (PGK1 reporter in pRIP plasmid)
pJD0360 pRS316 none





Genbank_Plasmid_Maps/pJD347.gb I think this is pRS316
pJD0361 pRS316 wt control, targets G2811





Genbank_Plasmid_Maps/pJD347.gb pBL152 (wt control, targets methylation to G2811)
pJD0362 pRS316 targets U2950





Genbank_Plasmid_Maps/pJD347.gb pBL232 (targets methylation to U2950)
pJD0363 pRS316 targets A2967





Genbank_Plasmid_Maps/pJD347.gb pBL233 (targets methylation to A2967)
pJD0364 pRS316 targets C2817





Genbank_Plasmid_Maps/pJD347.gb pBL226 (targets methylation to C2817)
pJD0365-1 pJD359 Ty1 frameshift signal AmpR Ura3 PGK1


Genbank_Plasmid_Maps/pJD347.gb Tymuta/Tyy2b, shifts into stop
pJD0365-2 pJD359 Ty1 frameshift signal AmpR Ura3 PGK1


Genbank_Plasmid_Maps/pJD347.gb Ty3b/Ty4b shifts away from stop
pJD0365-3 pJD359 Ty1 frameshift signal AmpR Ura3 PGK1


Genbank_Plasmid_Maps/pJD347.gb Tymutab/Ty6b mutant slippery site
pJD0366-1 pJD214 remove inframe AUG AmpR




Genbank_Plasmid_Maps/pJD347.gb pJD214 plasmids were modified to remove downstream inframe AUG (part of NcoI site at beginning of luciferase), derived from pJD214-18
pJD0366-2 pJD214 remove inframe AUG AmpR




Genbank_Plasmid_Maps/pJD347.gb pJD214 plasmids were modified to remove downstream inframe AUG (part of NcoI site at beginning of luciferase), derived from pJD214-Rpsi
pJD0366-3 pJD214 remove inframe AUG AmpR




Genbank_Plasmid_Maps/pJD347.gb pJD214 plasmids were modified to remove downstream inframe AUG (part of NcoI site at beginning of luciferase), derived from pJD366-1
pJD0367 pBluescript SK+



3471

Genbank_Plasmid_Maps/pJD367.gb Created by Victoria Lilling Sept '02. "pBluescript SK+ with 5' and 3' ends of X cloned in (reversed in this format) -- looks like 'X' is a fragment of L-A
pJD0368 pRS304 UPF3 ORF AmpR Trp1
~6000

Genbank_Plasmid_Maps/pJD367.gb 287bp 5'UTR, 1164 UPF3 ORF, 387bp 3'UTR in pRS304, XhoI and BamHI
pJD0369 pRS306 UPF3 ORF AmpR Ura3
~6000

Genbank_Plasmid_Maps/pJD367.gb 287bp 5'UTR, 1164 UPF3 ORF, 387bp 3'UTR in pRS306, XhoI and BamHI
pJD0370 pRS314 RPL41A





Genbank_Plasmid_Maps/pJD367.gb "RPL41A gene in plasmid pRS314, sent by Jonathan R Warner"
pJD0371 pBluescript SK+ URA3 AmpR




Genbank_Plasmid_Maps/pJD367.gb I think this is only URA3 in pBluescript SK+
pJD0372 pJD210.Trp
HygrBR Trp



Genbank_Plasmid_Maps/pJD367.gb "U1487C substitution in Helix 44 of 18S RNA conferring Hygromycin B resistance (U1759C in yeast)"
pJD0373 pRS425 35S HygR from pJD372 HygBR




Genbank_Plasmid_Maps/pJD367.gb "35S HygR from pJD372 cut out with Not/Sal and cloned into pRS425"
pJD0374 pRS426 35S HygR from pJD372 HygBR Ura3



Genbank_Plasmid_Maps/pJD367.gb "35S HygR from pJD372 cut out with Not/Sal and cloned into pRS425"
pJD0375
Dual luciferase cassette AmpR Ura dual lux
Cen
pJD375.gb

pJD0376 pJD375 LA AmpR Ura3 dual lux 9329
ColE1 Genbank_Plasmid_Maps/pJD376.gb

pJD0377 pJD375 TY1 frameshift signal AmpR Ura3 dual lux

ColE1 Genbank_Plasmid_Maps/pJD376.gb

pJD0378 pJD375 HIV-1 frameshift signal AmpR Ura3 dual lux


Genbank_Plasmid_Maps/pJD376.gb

pJD0379 pJD375 Ty3 frameshift signal AmpR Ura3 dual lux


Genbank_Plasmid_Maps/pJD376.gb

pJD0380 pRY20 HO
Ura3



Genbank_Plasmid_Maps/pJD376.gb pGal-HO is a mating type switch plasmid which came from the Methods in Enzymology, Vol 194 from 1991
pJD0381 pRS406

ura3



Genbank_Plasmid_Maps/pJD376.gb ATCC delta0 deleter vector set, 87468 -- pAD1
pJD0382 pRS406

ura3



Genbank_Plasmid_Maps/pJD376.gb ATCC delta0 deleter set, 87469 -- pAD2
pJD0383 pRS406

ura3



Genbank_Plasmid_Maps/pJD376.gb ATCC deleter set, 87470 -- pAD4
pJD0384 pRS305

leu2



Genbank_Plasmid_Maps/pJD376.gb ATCC deleter set, ura3 87471 -- pJL164
pJD0385 pRS406

ura3



Genbank_Plasmid_Maps/pJD376.gb ATCC deleter set, 87624 -- pNKY1009
pJD0386 pRS406

ura3



Genbank_Plasmid_Maps/pJD376.gb ATCC deleter set, 99604 -- pdeltaADE2
pJD0387 pJD375 premature termination codon AmpR Ura3 dual luc


Genbank_Plasmid_Maps/pJD376.gb "Yeast dual luciferase, out of frame control"
pJD0388 pRS413 GPD promoter
his3
5876 cen6
Genbank_Plasmid_Maps/pJD376.gb atcc 87354
pJD0389 pRS423 GPD promoter
his3
6709 2u
Genbank_Plasmid_Maps/pJD376.gb atcc 87355
pJD0390 pRS414 GPD promoter
trp1
5694 cen6
Genbank_Plasmid_Maps/pJD376.gb ATCC 87356
pJD0391 pRS424 GPD promoter
trp1
6527 2u
Genbank_Plasmid_Maps/pJD376.gb ATCC 87357
pJD0392 pRS415 GPD promoter
leu2
6927 cen6
Genbank_Plasmid_Maps/pJD376.gb ATCC 87358
pJD0393 pRS425 GPD promoter
leu2
7760 2u
Genbank_Plasmid_Maps/pJD376.gb ATCC 87359
pJD0394 pRS416 GPD promoter
ura3
5804 cen6
Genbank_Plasmid_Maps/pJD376.gb ATCC 87360
pJD0395 pRS426 GPD promoter
ura3
6637 2u
Genbank_Plasmid_Maps/pJD376.gb ATCC 87361
pJD0396 pRS413 TEF promoter
his3
5598 cen6
Genbank_Plasmid_Maps/pJD376.gb ATCC 87362
pJD0397 pRS423 TEF promoter
his3
6431 2u
Genbank_Plasmid_Maps/pJD376.gb ATCC 87363
pJD0398 pRS414 TEF promoter
trp1
5416 cen6
Genbank_Plasmid_Maps/pJD376.gb ATCC 87364
pJD0399 pRS424 TEF promoter
trp1
6249 2u
Genbank_Plasmid_Maps/pJD376.gb ATCC 87365
pJD0400 pRS415 TEF promoter
leu2
6649 cen6
Genbank_Plasmid_Maps/pJD376.gb ATCC 87366
pJD0401 pRS425 TEF promoter
leu2
7482 2u
Genbank_Plasmid_Maps/pJD376.gb ATCC 87367
pJD0402 pRS416 TEF promoter
ura3
5526 cen6
Genbank_Plasmid_Maps/pJD376.gb ATCC 87368
pJD0403 pRS426 TEF promoter
ura3
6359 2u
Genbank_Plasmid_Maps/pJD376.gb ATCC 87369
pJD0404 pRS413 ADH promoter
his3
6696 cen6
Genbank_Plasmid_Maps/pJD376.gb ATCC 87370
pJD0405 pRS423 ADH promoter
his3
7529 2u
Genbank_Plasmid_Maps/pJD376.gb ATCC 87371
pJD0406 pRS414 ADH promoter
trp1
6514 2u
Genbank_Plasmid_Maps/pJD376.gb ATCC 87372
pJD0407 pRS424 ADH promoter
trp1
7347 2u
Genbank_Plasmid_Maps/pJD376.gb ATCC 87373
pJD0408 pRS415 AHD promoter
leu2
7747 cen6
Genbank_Plasmid_Maps/pJD376.gb ATCC 87374
pJD0409 pRS416 ADH promoter
ura3
6624 cen6
Genbank_Plasmid_Maps/pJD376.gb ATCC 87376
pJD0410 pRS426 ADH promoter
ura3
7457 2u
Genbank_Plasmid_Maps/pJD420.gb ATCC 87377
pJD0411 pRS413 CYC1 promoter
his3
5483 cen6
pJD411.gb ATCC 87378
pJD0412 pRS423 CYC1 promoter
his3
6316 2u
Genbank_Plasmid_Maps/pJD420.gb ATCC 87379
pJD0413 pRS414 CYC1 promoter
trp1
5301 cen6
Genbank_Plasmid_Maps/pJD420.gb ATCC 87380
pJD0414 pRS424 CYC1 promoter
trp1
6134 2u
Genbank_Plasmid_Maps/pJD420.gb ATCC 87381
pJD0415 pRS415 CYC1 promoter
leu2
6534 cen6
pJD415.gb ATCC 87382
pJD0416 pRS425 CYC1 promoter
leu2
7367 2u
pJD416.gb ATCC 87383
pJD0417 pRS416 CYC1 promoter
ura3
5411 cen6
pJD417.gb ATCC 87384
pJD0418 pRS426 CYC1 promoter
ura3
6244 2u
pJD418.gb ATCC 87385
pJD0419 pRS415 O frame AmpR Leu2 dual lux 8658

pJD419.gb

pJD0419 pJD408 0 frame control
leu2 dual luciferase
cen6
pJD419.gb

pJD0420 pJD408 L-A frameshift signal
leu2 dual luciferase
cen6
Genbank_Plasmid_Maps/pJD420.gb

pJD0421 pJD408 TY1 frameshift signal
leu2 dual luciferase
cen6
Genbank_Plasmid_Maps/pJD420.gb

pJD0422 pCM182

trp1
7407

Genbank_Plasmid_Maps/pJD422.gb This is pCM182
pJD0423 pCM183

trp1
7512

Genbank_Plasmid_Maps/pJD423.gb This is pCM183
pJD0424 pCM184

trp1
7625

Genbank_Plasmid_Maps/pJD424.gb This is pCM184
pJD0426 pCM188

ura3
8156

Genbank_Plasmid_Maps/pJD426.gb This is pCM188
pJD0427 pCM189

ura3
8374

Genbank_Plasmid_Maps/pJD427.gb This is pCM189
pJD0428 TetOn/Off == pcM184
AmpR Trp

Cen6
Genbank_Plasmid_Maps/pJD420.gb

pJD0428 pCM190

ura3
8012

Genbank_Plasmid_Maps/pJD428.gb This is pCM190
pJD0428a pJD428 / pRS426

ura3
7794

Genbank_Plasmid_Maps/pJD420.gb

pJD0428b pJD428 / pJD427

ura3
8012

Genbank_Plasmid_Maps/pJD420.gb This is a replacement for pJD428
pJD0429 pRS426 RPL10 AmpR Ura3
6729 2u
Genbank_Plasmid_Maps/pJD429.gb

pJD0430
Aptamer





Genbank_Plasmid_Maps/pJD420.gb Construct made by Bani
pJD0431 pJD375 UAA in firefly luciferase AmpR Ura3 dual lux 9224

Genbank_Plasmid_Maps/pJD431.gb

pJD0432 pJD375 UAG in firefly luciferase AmpR Ura3 dual lux 9224

Genbank_Plasmid_Maps/pJD432.gb

pJD0433 pJD375 UGA in firefly luciferase AmpR Ura3 dual lux 9224

Genbank_Plasmid_Maps/pJD433.gb

pJD0435 pJD175e SARS frameshift signal AmpR
dual lux 5479

Genbank_Plasmid_Maps/pJD435.gb

pJD0436 pJD409 SARS frameshift signal AmpR Ura3 dual lux


Genbank_Plasmid_Maps/pJD435.gb SARS1 was inserted at spe1/xho1 site
pJD0437 pJD409 SARS frameshift mutant AmpR




Genbank_Plasmid_Maps/pJD435.gb SDM was don’t at stem 3 SARS-1 frameshift.
pJD0438 pJD409 SARS frameshift mutant AmpR




Genbank_Plasmid_Maps/pJD435.gb SDM was done at stem 1 of SARS-1 frameshift.
pJD0439 pJD409 SARS frameshift mutant AmpR




Genbank_Plasmid_Maps/pJD435.gb SDM was done at stem 2 of SARS-1 frameshift.
pJD0440 pJD409 SARS frameshift mutant AmpR




Genbank_Plasmid_Maps/pJD435.gb SDM was done at stem 4 of SARS-1 frameshift.
pJD0441 pUG23 yEGFP3 AmpR HIS3
6303

Genbank_Plasmid_Maps/pJD441.gb

pJD0442 pUG34 yEGFP3 AmpR HIS3
6297

Genbank_Plasmid_Maps/pJD442.gb

pJD0443 pUG35
AmpR Ura3
6231

Genbank_Plasmid_Maps/pJD443.gb This is pUG35
pJD0444 pUG36 yEGFP3 AmpR HIS3
6335

Genbank_Plasmid_Maps/pJD444.gb

pJD0445 pJD409 SARS-1 in frame





Genbank_Plasmid_Maps/pJD435.gb SARS-1, in frame
pJD0446 pJD437 SARS stem 3 is mutagenized AmpR




Genbank_Plasmid_Maps/pJD435.gb SARS-1 stem 3 is mutagenized and -1 frame is correct. Original pJD437 in 0 frame
pJD0447 pJD438 SARS stem 1 mutagenized AmpR




Genbank_Plasmid_Maps/pJD435.gb SARS-1 stem 1 is mutagenized and -1 frame is correct original pJD438 in 0 frame
pJD0448 pJD175f SARS pseudoknot AmpR

5479

Genbank_Plasmid_Maps/pJD448.gb SARS pseudoknot in pJD175f
pJD0449 pJD375 SARS pseudoknot AmpR Ura3
9301

Genbank_Plasmid_Maps/pJD449.gb

pJD0450

AmpR

4242

Genbank_Plasmid_Maps/pJD450d.gb

pJD0452
SARS-1 stem4 mutated, double mutant





Genbank_Plasmid_Maps/pJD450d.gb "SARS-1, stem no 4 was mutated, ie double mutant."
pJD0453







Genbank_Plasmid_Maps/pJD450d.gb Aptamer sequence flanked by Bpu1102I
pJD0454







Genbank_Plasmid_Maps/pJD450d.gb Insertion of two nt (AT) before Bpu1102I site by SDM
pJD0455







Genbank_Plasmid_Maps/pJD450d.gb Insertion of 5 nt (ATCAT) after Bpu1102I site by SDM
pJD0456 pJD175 pseudo pseudo knot AmpR

5488

Genbank_Plasmid_Maps/pJD456.gb "Pseudo pseudo reverse knot fluc out of frame"
pJD0457 pJD175 pseudo pseudo knot stem loop fluc out of frame AmpR

5488

Genbank_Plasmid_Maps/pJD457.gb "Pseudo pseudo knot stem loop fluc out of frame"
pJD0458 pJD175 pseudo pseudoknot AmpR

5488

Genbank_Plasmid_Maps/pJD458.gb pseudo pseudonot readthrough fluc out of frame
pJD0459 pJD175 pseudo pseudoknot AmpR

5488

Genbank_Plasmid_Maps/pJD459.gb pseudo pseudoknoy alternate stem fluc out of frame
pJD0460 pJD175 pseudo pseudoknot AmpR

5492

Genbank_Plasmid_Maps/pJD460.gb XhoI digest and end fill of pJD456, 4 base insertion
pJD0461 pJD457 psedoknot reverse knot 4nt AmpR Ura3 dual lux 5492
ColE1 Genbank_Plasmid_Maps/pJD461.gb XhoI digest and endfill of pJD457 = 4 nt insertion, Rluc 0 frame at BamHI site, slippery site @ -1 frame, fluc in -1 frame at SacI
pJD0463 pJD175 pseudo pseudoknot AmpR

5505

Genbank_Plasmid_Maps/pJD463.gb

pJD0464 pJD175 Wild-type SARS PRF Control Amp
dual luciferase

ColE1/ Genbank_Plasmid_Maps/pJD463.gb Ewan
pJD0465 pJD375 SARS -1 frame AmpR Ura3
9274

Genbank_Plasmid_Maps/pJD465.gb

pJD0467 pJD375 S3A SARS AmpR Ura3
9302

Genbank_Plasmid_Maps/pJD467.gb

pJD0469 pJD375 S3 delta SARS AmpR Ura3
9284

Genbank_Plasmid_Maps/pJD469.gb

pJD0471







Genbank_Plasmid_Maps/pJD463.gb Aptamer mutant construction was made by bani
pJD0472







Genbank_Plasmid_Maps/pJD472.gb BamHI/EcoRI fragment from pJD346 cloned into pJD175f, then fluc replaced by cloning SacI/EcoRI from pJD449 -- these instructions make no sense.
pJD0473




5504

Genbank_Plasmid_Maps/pJD473.gb

pJD0474







Genbank_Plasmid_Maps/pJD463.gb two cc was added before ggatcc site, with one a added at fluc1724 position to correct the readthrough frame
pJD0475 pJD472? S3D





Genbank_Plasmid_Maps/pJD463.gb to make the original -1 frame one c was added at bamh1 site through sdm
pJD0476 pJD375 PPR1 PRF





Genbank_Plasmid_Maps/pJD476.gb

pJD0477 pJD375 NUP82 PRF





Genbank_Plasmid_Maps/pJD477.gb

pJD0478 pJD375 TBF1 PRF





Genbank_Plasmid_Maps/pJD478.gb

pJD0479
SARS S3b





Genbank_Plasmid_Maps/pJD463.gb new mutnt of SARS, mutant in 1b, construct made by bani, s3b
pJD0480
SARS S2b





Genbank_Plasmid_Maps/pJD463.gb new mutant of SARS, mutant made in 3b, construct by bani, replaced with a new construct with no termination codon in frame
pJD0481 p416 RFP AmpR Ura3
6624

Genbank_Plasmid_Maps/pJD463.gb Promoter-xba1-spe1-RFP-BamHI-SmaI-EcoRV-EcoRI-PstI-HindIII-ClaI-SalI-XhoI-Terminator
pJD0482 p416 CFP AmpR Ura3
6624

Genbank_Plasmid_Maps/pJD463.gb Promoter-xba1-spe1-CFP-BamHI-SmaI-EcoRV-EcoRI-PstI-HindIII-ClaI-SalI-XhoI-Terminator
pJD0484 pJD375 YMR269 PRF AmpR Ura3
9259

Genbank_Plasmid_Maps/pJD484.gb This was in the wrong reading frame due to a misannotation in the SGD
pJD0486 pJD480/pJD175e S2B





Genbank_Plasmid_Maps/pJD486.gb

pJD0487 pJD467/pJD175e S3A





Genbank_Plasmid_Maps/pJD487.gb

pJD0488 pJD479/pJD175e S3B





Genbank_Plasmid_Maps/pJD488.gb

pJD0489 pJD475/pJD175e S3C





Genbank_Plasmid_Maps/pJD489.gb

pJD0490 pJD469/pJD175e Delta53





Genbank_Plasmid_Maps/pJD490.gb

pJD0491
New S2C





Genbank_Plasmid_Maps/pJD490.gb

pJD0492
New S3C





Genbank_Plasmid_Maps/pJD490.gb

pJD0493 p416 RFP LA CFP AmpR Ura3
6624

Genbank_Plasmid_Maps/pJD490.gb speI-RFP-LA-FS-CFP-XhoI
pJD0494







Genbank_Plasmid_Maps/pJD490.gb "SK+ w/ PTC from KasI to pFIMI inserted with XhoI and HindIII"
pJD0495 pBluescript YLR014





Genbank_Plasmid_Maps/pJD490.gb

pJD0496 pBluescript YJL061





Genbank_Plasmid_Maps/pJD490.gb

pJD0497 pBluescript YGL103





Genbank_Plasmid_Maps/pJD490.gb

pJD0498 pBluescript YPL128





Genbank_Plasmid_Maps/pJD490.gb

pJD0499 pBluescript YMR269





Genbank_Plasmid_Maps/pJD490.gb

pJD0500 pBluescript YDR371





Genbank_Plasmid_Maps/pJD490.gb

pJD0501 pJD175e 0 frame SARS





Genbank_Plasmid_Maps/pJD490.gb

pJD0502 pJD175 Wild-type SARS PRF test Amp
dual luciferase

ColE1/ Genbank_Plasmid_Maps/pJD502.gb Ewan
pJD0503 pJD175e S2A





Genbank_Plasmid_Maps/pJD503.gb

pJD0504 pJD175e S2C





Genbank_Plasmid_Maps/pJD504.gb

pJD0505 pJD175e S2D





Genbank_Plasmid_Maps/pJD505.gb

pJD0506 pJD175e S3C





Genbank_Plasmid_Maps/pJD506.gb

pJD0507 pSMART-kan SARS clone D





Genbank_Plasmid_Maps/pJD490.gb Comes from yount et al. 2003
pJD0508







Genbank_Plasmid_Maps/pJD490.gb Subclone at MluI-NdeI from pJD453+HygR
pJD0509







Genbank_Plasmid_Maps/pJD490.gb Subclone at MluI-NdeI from pJD430+HygR
pJD0510







Genbank_Plasmid_Maps/pJD490.gb Subclone at MluI-NdeI from pJD454+HygR
pJD0512 pJD175f YPL128T (TBF1)





Genbank_Plasmid_Maps/pJD512.gb

pJD0513 pJD175f YOR026W (BUB3)





Genbank_Plasmid_Maps/pJD513.gb

pJD0514 pJD175d YER115C (SPR6)





Genbank_Plasmid_Maps/pJD514.gb

pJD0515 pJD175f YLR318W (EST2)





Genbank_Plasmid_Maps/pJD515.gb

pJD0516 pJD175f YBR008C (FLR1)





Genbank_Plasmid_Maps/pJD516.gb

pJD0517 pJD175f YLR342W (FKS1)





Genbank_Plasmid_Maps/pJD517.gb

pJD0518 pJD375 YPL128T





Genbank_Plasmid_Maps/pJD518.gb

pJD0519 pJD375 YOR026W (BUB3)





Genbank_Plasmid_Maps/pJD519.gb

pJD0520 pJD375 YER115C





Genbank_Plasmid_Maps/pJD520.gb

pJD0521 pJD375 YLR318W





Genbank_Plasmid_Maps/pJD521.gb

pJD0522 pJD375 YBR008C





Genbank_Plasmid_Maps/pJD522.gb

pJD0523 pJD375 YLR342W (FKS1)





Genbank_Plasmid_Maps/pJD523.gb

pJD0524 pJD507 a13395g, c1198c/a KanR




Genbank_Plasmid_Maps/pJD524.gb

pJD0525 pJD507 13305g, c13309c/a KanR




Genbank_Plasmid_Maps/pJD525.gb

pJD0526 pJD507 a13395-7t, c13398c/t KanR




Genbank_Plasmid_Maps/pJD526.gb

pJD0527 pJD507 a13395-7t, c13398c/t KanR




Genbank_Plasmid_Maps/pJD527.gb

pJD0528 pJD507 t13392-4g KanR




Genbank_Plasmid_Maps/pJD528.gb

pJD0529 pJD507 t13392-4g KanR




Genbank_Plasmid_Maps/pJD529.gb

pJD0530 pJD507 a13395-75, c13398a KanR




Genbank_Plasmid_Maps/pJD530.gb

pJD0531 pJD507 t13392-4g, a13395-7t, c13398a KanR




Genbank_Plasmid_Maps/pJD531.gb

pJD0532 pJD507 t13392-4a KanR




Genbank_Plasmid_Maps/pJD532.gb

pJD0533 pJD507 a13395-7t, c13398c/t KanR




Genbank_Plasmid_Maps/pJD533.gb

pJD0534 pJD507 a13395-7t, c13398c/t KanR




Genbank_Plasmid_Maps/pJD534.gb

pJD0535 pJD507 a13395-7t, c13398c/t KanR




Genbank_Plasmid_Maps/pJD535.gb

pJD0536 p2luc SARS pseudoknot, GC swap 3' half of stem3 AmpR none dual luciferase 5480 none ColE1/ Genbank_Plasmid_Maps/pJD536.gb
Ewan
pJD0537 p2luc SARs pseudoknot A13467C AmpR none dual luciferase 5480 none ColE1/ Genbank_Plasmid_Maps/pJD537.gb A13467C in SARS pseudoknot (the bulged base in stem 2) Ewan
pJD0538 p2luc sars pseudoknot, gc swap and A13465G AmpR none dual luciferase 5480 none ColE1/ Genbank_Plasmid_Maps/pJD538.gb Has G/C swaps in 3' half of stem 2 of SARS pseudoknot, including A13465G Ewan
pJD0539 p2luc SARS pseudoknot AmpR none dual luciferase 5480 none ColE1/ Genbank_Plasmid_Maps/pJD539.gb A13456C in SARS pseudoknot (bulged residue in stem 3) Ewan
pJD0540 p2luc a13465g in sars pseudoknot AmpR none dual luciferase 5480 none ColE1/ Genbank_Plasmid_Maps/pJD540.gb A13465G in SARS pseudoknot (changing base in the loop between 3' stem 3 and 3' stem 2 to prevent stop codon forming when subsequent stem 2 mutations are made) Ewan
pJD0541 p2luc gc swap in both portions of stem2, a13465g sars pseudoknot AmpR none dual luciferase 5480 none ColE1/ Genbank_Plasmid_Maps/pJD540.gb Has G/C waps in both 5' and 3' portions of stem 2 of the SARS pseudoknot, including A13465G Ewan
pJD0542 pJD469 BamHI/EcoRI from pJD537 into 469 ampR Ura3 dual lux


Genbank_Plasmid_Maps/pJD540.gb

pJD0543 pJD469 BamHI/EcoRI from 538 into 469 ampR Ura3 dual lux


Genbank_Plasmid_Maps/pJD540.gb

pJD0543 pJD469 SARS pseudoknot AmpR URA3 dual luciferase 9274 /2u ColE1/ Genbank_Plasmid_Maps/pJD540.gb BamH1/EcoR1 fragment of pJD538 cloned into pJD469 Ewan
pJD0544 pJD469 SARS pseudoknot AmpR URA3 dual luciferse 9274 /2u ColE1/ Genbank_Plasmid_Maps/pJD540.gb BamH1/EcoR1 fragment of pJD539 cloned into pJD469 Ewan
pJD0545 pJD469 SARS pseudoknot AmpR URA3 dual luciferase 9274 /2u ColE1/ Genbank_Plasmid_Maps/pJD540.gb BamH1/EcoR1 fragment of pJD540 cloned into pJD469 Ewan
pJD0546 pJD469 SARS pseudoknot AmpR URA3 dual luciferase 9274 /2u ColE1/ Genbank_Plasmid_Maps/pJD540.gb BamH1/EcoR1 fragment of pJD541 cloned into pJD469 Ewan
pJD0547
PAP mutant, Nterminal domain, NT U13
Leu2



Genbank_Plasmid_Maps/pJD540.gb

pJD0548
PAP mutant, Nterminal domain, NT 5U2
Leu2



Genbank_Plasmid_Maps/pJD540.gb

pJD0549
PAP mutant, Nterminal domain, NT 5U8
Leu2



Genbank_Plasmid_Maps/pJD540.gb

pJD0550
Central domain mutant: NT 502
Leu2



Genbank_Plasmid_Maps/pJD540.gb

pJD0551
Central domain mutant: NT 501
Leu2



Genbank_Plasmid_Maps/pJD540.gb

pJD0552
Central domain mutant: NT 538
Leu2



Genbank_Plasmid_Maps/pJD540.gb

pJD0553
Central domain mutant: NT 532
Leu2



Genbank_Plasmid_Maps/pJD540.gb

pJD0554
Central domain mutant: NT 242
Leu2



Genbank_Plasmid_Maps/pJD540.gb

pJD0555
Central domain mutant: NT 483
Leu2



Genbank_Plasmid_Maps/pJD540.gb

pJD0556
Central domain mutant: NT 485
Leu2



Genbank_Plasmid_Maps/pJD540.gb

pJD0557
C terminal domain mutation: NT 333
Leu2



Genbank_Plasmid_Maps/pJD540.gb

pJD0558
C terminal domain mutation: NT 486
Leu2



Genbank_Plasmid_Maps/pJD540.gb

pJD0559
C terminal domain mutation: NT 420
Leu2



Genbank_Plasmid_Maps/pJD540.gb

pJD0560
C terminal domain mutation: NT 456
Leu2



Genbank_Plasmid_Maps/pJD540.gb

pJD0561
Vector: NT 188
Leu2



Genbank_Plasmid_Maps/pJD540.gb

pJD0562
telomerase mutant





Genbank_Plasmid_Maps/pJD540.gb

pJD0563
telomerase mutant





Genbank_Plasmid_Maps/pJD540.gb

pJD0564
telomerase mutant





Genbank_Plasmid_Maps/pJD540.gb

pJD0565
telomerase mutant





Genbank_Plasmid_Maps/pJD540.gb

pJD0566
telomerase mutant





Genbank_Plasmid_Maps/pJD540.gb

pJD0567 pEP-A 3' stem 3 SARS mutant
Ura dual lux


Genbank_Plasmid_Maps/pJD540.gb Mutants of SARS between Renilla/firefly
pJD0568 pEP-A mut 1.1 U UUG AAC
Ura dual lux


Genbank_Plasmid_Maps/pJD540.gb Mutants of SARS between Renilla/firefly
pJD0569 pEP-A mut 1.2 U UUG AAA
Ura dual lux


Genbank_Plasmid_Maps/pJD540.gb Mutants of SARS between Renilla/firefly
pJD0570 pEP-A mut 2.1 U UUU UUU
Ura dual lux


Genbank_Plasmid_Maps/pJD540.gb Mutants of SARS between Renilla/firefly
pJD0571 pEP-A mut 2.2 U UUU UUC
Ura dual lux


Genbank_Plasmid_Maps/pJD540.gb Mutants of SARS between Renilla/firefly
pJD0572 pEP-A mut 3 G GGA AAC
Ura dual lux


Genbank_Plasmid_Maps/pJD540.gb Mutants of SARS between Renilla/firefly
pJD0573
mut 4 G GGG GGG

dual lux


Genbank_Plasmid_Maps/pJD540.gb Mutants of SARS between Renilla/firefly
pJD0574
5.1 G GGU UUU

dual lux


Genbank_Plasmid_Maps/pJD540.gb Mutants of SARS between Renilla/firefly
pJD0575 pEP-A 5.2 G GGU UUA
Ura dual lux


Genbank_Plasmid_Maps/pJD540.gb Mutants of SARS between Renilla/firefly
pJD0576 pEP-A 5.3 G GGU UUC
Ura dual lux


Genbank_Plasmid_Maps/pJD540.gb Mutants of SARS between Renilla/firefly
pJD0577 pEP-A 5.4 G GGU UUG
Ura dual lux


Genbank_Plasmid_Maps/pJD540.gb Mutants of SARS between Renilla/firefly
pJD0578 pEP-A 6 A AAA AAU
Ura dual lux


Genbank_Plasmid_Maps/pJD540.gb Mutants of SARS between Renilla/firefly – causes problems and stop codons.
pJD0579 pJD375 insert bamhi site into pJD375
Ura3 dual lux


Genbank_Plasmid_Maps/pJD540.gb

pJD0580 pJD375 insert ga into pJD375
Ura3 dual lux


Genbank_Plasmid_Maps/pJD540.gb ga insertion creates +1 frame at 3942
pJD0581 pJD175e BamH1/EcoR1 from pJD568 (sars with mut slipsite)

dual lux


Genbank_Plasmid_Maps/pJD540.gb

pJD0582 pJD175e SARS mut slipsite UUUGAAA from 569

dual lux


Genbank_Plasmid_Maps/pJD540.gb

pJD0583 pJD175e EcoR1/BamH1 from 570

dual lux


Genbank_Plasmid_Maps/pJD540.gb

pJD0584 pJD175e EcoR1/BamH1 from 578

dual lux


Genbank_Plasmid_Maps/pJD540.gb

pJD0585 pJD584 Sars slipsite A AAU UUA SDM of 584

dual lux


Genbank_Plasmid_Maps/pJD540.gb

pJD0586 pRS313 RPL11b into pRS313
His3

Cen
Genbank_Plasmid_Maps/pJD540.gb cloning appears to be between sac2/EcoR1
pJD0587 pRS314 RPL11b into pRS314
Trp1

Cen
Genbank_Plasmid_Maps/pJD540.gb cloning appears to be between sac2/EcoR1
pJD0588 pRS316 RPL11b into pRS316
Ura3

Cen
Genbank_Plasmid_Maps/pJD540.gb cloning appears to be between sac2/EcoR1
pJD0589 pRS313 RPL10 insert
His3

Cen
Genbank_Plasmid_Maps/pJD589.gb Between Stu1 sites
pJD0590 pJD508 RDN18, HygR, RDN58, RDN25, RDN5 AmpR Trp1 lacZ
2 micron
Genbank_Plasmid_Maps/pJD540.gb mutant C-G – 3020nt of 25S
pJD0591 pJD508 RDN18, HygR, RDN58, RDN25, RDN5 AmpR Trp1 lacZ
2 micron
Genbank_Plasmid_Maps/pJD540.gb mutant C-A 3020 nt of 25S
pJD0592 pJD508 RDN18, HygR, RDN58, RDN25, RDN5 AmpR Trp1 lacZ
2 micron
Genbank_Plasmid_Maps/pJD540.gb mutant C-T 3020 nt of 25S
pJD0593 pJD508 RDN18, HygR, RDN58, RDN25, RDN5 AmpR Trp1 lacZ
2 micron
Genbank_Plasmid_Maps/pJD540.gb mutant G-A 3020 nt of 25S
pJD0594 pJD508 RDN18, HygR, RDN58, RDN25, RDN5 AmpR Trp1 lacZ
2 micron
Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0595 pJD508 RDN18, HygR, RDN58, RDN25, RDN5 AmpR Trp1 lacZ
2 micron
Genbank_Plasmid_Maps/pJD540.gb mutant G-C 3020 nt of 25S
pJD0596 pJD508 RDN18, HygR, RDN58, RDN25, RDN5 AmpR Trp1 lacZ
2 micron
Genbank_Plasmid_Maps/pJD540.gb mutant A-G 3020 nt of 25s
pJD0597 pJD508 RDN18, HygR, RDN58, RDN25, RDN5 AmpR Trp1 lacZ
2 micron
Genbank_Plasmid_Maps/pJD540.gb mutant G-A 3020 nt of 25S
pJD0598 pJD508 RDN18, HygR, RDN58, RDN25, RDN5 AmpR Trp1 lacZ
2 micron
Genbank_Plasmid_Maps/pJD540.gb mutant G-C 3020 nt of 25S
pJD0599 pJD508 RDN18, HygR, RDN58, RDN25, RDN5 AmpR Trp1 lacZ
2 micron
Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0600 pBluescript SK+ Mutant A—G





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0601 pBluescript SK+ Mutant A—T





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0602 pBluescript SK+ mutant A—C





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0603 pBluescript SK+ mutant C—G





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0604 pBluescript SK+ mutant C—T





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0605 pBluescript SK+ mutant G—A





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0606 pBluescript SK+ mutant G—T





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0607 pBluescript SK+ mutant G—C





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0608 pBluescript SK+ mutant A—C





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0609 pBluescript SK+ Mutant A—T





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0610 pBluescript SK+ Mutant A—G





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0611 pBluescript SK+ mutant G—A





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0612 pBluescript SK+ mutant G—C





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0613 pBluescript SK+ mutant G—T





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0614 pBluescript SK+ Mutant A—T





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0615 pBluescript SK+ mutant A—C





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0616 pBluescript SK+ Mutant A—G





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0617 pBluescript SK+ Mutant A—G





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0618 pBluescript SK+ mutant G—A





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0619 pBluescript SK+ mutnat G—C





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0620 pBluescript SK+ mutant G—T





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0621 pBluescript SK+ mutant G—T





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0622 pBluescript SK+ mutant G—A





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0623 pBluescript SK+ mutant G—C





Genbank_Plasmid_Maps/pJD540.gb mutant G-T 3020 nt of 25S
pJD0624 pJD477 nup82 in pJD375 AmpR Ura3 dual lux
Cen
Genbank_Plasmid_Maps/pJD540.gb single a inserted between renilla/firefly after prf signal dinman
pJD0625 pJD485 CTS2 in pJD375 AmpR Ura3 dual lux
Cen
Genbank_Plasmid_Maps/pJD540.gb T between renilla/firefly in spacer region
pJD0626 pJD520 SPR6 in pJD375 AmpR Ura3 dual lux
Cen
Genbank_Plasmid_Maps/pJD540.gb A between Renilla/Ffly
pJD0627 pRS313 +RPL2A





Genbank_Plasmid_Maps/pJD540.gb

pJD0628 pRS315 +RPL2A





Genbank_Plasmid_Maps/pJD540.gb

pJD0629 pRS316 +RPL2A





Genbank_Plasmid_Maps/pJD540.gb

pJD0630 pRS313 +RPL4a





Genbank_Plasmid_Maps/pJD540.gb

pJD0631 pRS315 +RPL4a





Genbank_Plasmid_Maps/pJD540.gb

pJD0632 pRS316 +RPL4a





Genbank_Plasmid_Maps/pJD540.gb

pJD0633 pJD406 dual lux 0 frame
Trp dual lux


Genbank_Plasmid_Maps/pJD540.gb

pJD0634 pJD406 dual lux LA
Trp dual lux


Genbank_Plasmid_Maps/pJD540.gb

pJD0635 pJD406 dual lux Ty1
Trp dual lux


Genbank_Plasmid_Maps/pJD540.gb

pJD0636 pJD406 dual lux HIVI
Trp dual lux


Genbank_Plasmid_Maps/pJD540.gb

pJD0637
EST1/Ura3? AmpR




Genbank_Plasmid_Maps/pJD540.gb pVL157 EST1 fragment in BamH1/Sph1 digestions of Yep24
pJD0638 yep lac181 Est2/Leu2 AmpR Leu2

2 micron
Genbank_Plasmid_Maps/pJD638-EST2.gb EST2 coding sequence including UTRs from BamH1/Sac1 sites.
pJD0639 yep lac181 EST3/Leu2 AmpR Leu2

2 micron
Genbank_Plasmid_Maps/pJD540.gb 1.15kb fragment from EST3
pJD0640 yep lac181 STN1/Leu2 AmpR Leu2

2 micron
Genbank_Plasmid_Maps/pJD540.gb STN1 orf + ~300bp upstream, downstream includes sORF
pJD0641 pJD638, pRS315 EST2 / Leu2 ampR Leu2
9738 Cen6 ColE1 Genbank_Plasmid_Maps/pJD641.gb restriction digestion of pJD638 with BamHI and SacI and sub cloned into pRS315 Jonathan Jacobs
pJD0642 pJD375 mutant R218S in firefly luciferase ampR URA3 dual luciferase 9221
ColE1 Genbank_Plasmid_Maps/pJD641.gb non-cognate TCT codon Ewan
pJD0643 pJD375 R218S mutant in firefly luciferase ampR URA3 dual luciferase 9221
ColE1 Genbank_Plasmid_Maps/pJD641.gb near-cognate AGC codon Ewan
pJD0644 pJD375 R218S mutant in firefly luciferase ampR URA3 dual luciferase 9221
ColE1 Genbank_Plasmid_Maps/pJD641.gb near-cognate AGT codon Ewan
pJD0645 pJD175e / pJD572 SARS slipsite mutant (U UUU UUC) AmpR
dual luciferase 5480
ColE1 Genbank_Plasmid_Maps/pJD641.gb SARS slippery site mutant (U UUU UUC) made by subcloning the EcoRI/BamHI fragment from pJD571 into pJD175e
pJD0646 pJD175e / pJD572 SARS slipsite mutant (G GGA AAC) AmpR
dual luciferase 5480
ColE1 Genbank_Plasmid_Maps/pJD641.gb SARS slippery site mutant (G GGA AAC) made by subcloning the EcoRI/BamHI fragment from pJD571 into pJD175e
pJD0647 pJD175e / pJD575 SARS slipsite mutant (G GGU UUA) AmpR
dual luciferase 5480
ColE1 Genbank_Plasmid_Maps/pJD641.gb Sars slipsite mutant (G GGU UUA) made by subcloning EcoRII/BamHI fragment from pJD575 into pJD175e
pJD0648 pJD175e / pJD576 SARS slipsite mutant (G GGU UUC) AmpR
dual luciferase 5480
ColE1 Genbank_Plasmid_Maps/pJD641.gb Sars slipsite mutant (G GGU UUC) made by subcloning the EcoRI/BamHI fragment from pJD576 into pJD175e
pJD0649 pJD175e / pJD577 Sars slipsite mutant (G GGU UUG) AmpR
dual luciferase 5480
ColE1 Genbank_Plasmid_Maps/pJD641.gb Sars slipsite mutant (G GGU UUG) made by subcloning the EcoRI/BamHI fragment from pJD577 into 175e
pJD0650 pJD375 R218S mutation of firefly luciferase AmpR URA3 dual luciferase 9221 2u ColE1 Genbank_Plasmid_Maps/pJD641.gb R218S mutation in firefly luciferase was made by SDM and confirmed by sequencing, non-cognate TCC codon
pJD0651 pJD375 mutant R218C in firefly luciferase AmpR URA3 dual luciferase 9221 2u ColE1 Genbank_Plasmid_Maps/pJD641.gb The R218C mutation in firefly luciferase was made by site directed mutagenesis on pJD375, this has non cognate TGT codon Ewan
pJD0652 pJD375 mutant R218T in firefly luciferase AmpR URA3 dual luciferase 9221 2u ColE1 Genbank_Plasmid_Maps/pJD641.gb The R218T mutation in firefly luciferase was made by site directed mutagensis, this has non-cognate ACC codon Ewan
pJD0653 pJD375 mutant R218T in firefly luciferase AmpR URA3 dual luciferase 9221 2u ColE1 Genbank_Plasmid_Maps/pJD641.gb The R218T mutation in firefly lucfierase was made by site directed mutagenesis, this has the non-cognate ACT codon Ewan
pJD0654 pJD469/pJD584 BamHI fragment from pJD584 AmpR URA3 dual luciferase 9274 2u ColE1 Genbank_Plasmid_Maps/pJD641.gb Cloning the BamHI fragment from pJD584 into pJD469, has slippery site A AAA AAU Ewan
pJD0655 pJD469/pJD585 BamHI fragment from pJD585 AmpR URA3 dual luciferase 9274 2u ColE1 Genbank_Plasmid_Maps/pJD641.gb Cloning the BamHI fragment from pJD585 into pJD469, has slippery site A AAU UUA Ewan
pJD0656 pJD502
AmpR
dual luciferase 5480
ColE1 Genbank_Plasmid_Maps/pJD641.gb Site directed mutagenesis of pDJ502 to make slipsite C CCA AAC Ewan
pJD0657 pJD502
AmpR
dual luciferase 5480
ColE1 Genbank_Plasmid_Maps/pJD641.gb Site directed mutagenesis of pJD502 to make slipsite A AAA AAC Ewan
pJD0658 pJD502
AmpR
dual luciferase 5480
ColE1 Genbank_Plasmid_Maps/pJD641.gb site directed mutagenesis of pJD502 to make slipsite G GGU UUU Ewan
pJD0659 pJD375 EST2 12-183 (ss at 72) AmpR Ura dual lux
Cen6
Genbank_Plasmid_Maps/pJD659.gb

pJD0660 pJD375 EST2 1174-1305 (ss at 1215) AmpR Ura dual lux
Cen6
Genbank_Plasmid_Maps/pJD660.gb

pJD0661 pJD375 EST2 1284-1473 (1326) AmpR Ura dual lux
Cen6
Genbank_Plasmid_Maps/pJD661.gb

pJD0662 pJD375 EST2 1611-1800 (ss at1653) AmpR Ura dual lux
Cen6
Genbank_Plasmid_Maps/pJD662.gb

pJD0663

AmpR




Genbank_Plasmid_Maps/pJD662.gb "mCherry in AmpR vector, no map available." Alexey
pJD0664 pJD665 / pJD666 HIV PRF signal AmpR
dual fluorescence 4260
ColE1 Genbank_Plasmid_Maps/pJD662.gb "DFR HIV -1 Reporter, 'mammalian' expression vector Alexey
pJD0665
cyan fluorescent protein AmpR

3488
ColE1 Genbank_Plasmid_Maps/pJD662.gb looks like 1/2 of a DFR vector Alexey
pJD0666
mcherry fluorescent protein AmpR

3481
ColE1 Genbank_Plasmid_Maps/pJD662.gb looks like the other 1/2 of a DFR vector Alexey
pJD0667 pJD375 EST2 1956-2142 (ss at 1995) AmpR Ura dual lux
Cen6
Genbank_Plasmid_Maps/pJD667.gb

pJD0668 pJD375 BUB3 795-939 AmpR Ura dual lux
Cen6
Genbank_Plasmid_Maps/pJD662.gb

pJD0669 pJD375 SPR6 PRF signal (YER115C) AmpR URA3 dual luciferase 9428 2u ColE1 Genbank_Plasmid_Maps/pJD662.gb "The SPR6 subsequence was cloned into pJD375 after PCR amplification from the yeast genome" jonathan Jacobs
pJD0670 pJD375 TBF1 1488-1686 AmpR Ura dual lux
Cen6
Genbank_Plasmid_Maps/pJD662.gb

pJD0671 pJD375 CTS2 1155-1359 AmpR Ura dual lux
Cen6
Genbank_Plasmid_Maps/pJD662.gb

pJD0672 pJD375 NUP82 1494-1707 AmpR Ura dual lux
Cen6
Genbank_Plasmid_Maps/pJD662.gb

pJD0673 pJD641 EST2 full length slipsite disruption 1215 AmpR Leu2
9738 Cen6 ColE1 Genbank_Plasmid_Maps/pJD673.gb This plasmid is based on pJD641. It is a SDM of slippery site 1215 in EST2 whereby silent mutations were made to change the slippery site 1215 from acCCCTTTT to acTCCATTC; presumably abrogating potential PRF
pJD0674 pJD641 EST2 full length slipsite disruption 1995 AmpR Leu2
9738 Cen6 ColE1 Genbank_Plasmid_Maps/pJD674.gb This plasmid is based on pJd641. It is a SDM of slippery site 1995 in EST2 whereby silent mutations were made to change slippery site 1995 from atTTTAAAA to atATTGAAG, abrogating potential PRF
pJD0675 pJD665 / pJD666 Dual Flourescence Reporter; HIV 0 frame control AmpR
dual fluorescence 4261
ColE1 Genbank_Plasmid_Maps/pJD662.gb "Mammalian vector" -- a dual fluroescence mammalian expression vector with a 0 frame control for HIV Alexey
pJD0676 pJD642 non cognate Firefly luciferase (TCT at position 218) AmpR TRP1 dual luciferase 9111
ColE1 Genbank_Plasmid_Maps/pJD662.gb Has TCT at position 218, see pJD642
pJD0677 pJD643 firefly near cognate AmpR Trp1 dual luciferase 9111
ColE1 Genbank_Plasmid_Maps/pJD662.gb from pJD643 in trp vector pJD406 (trp 0 frame pJD633)
pJD0678 pJD465 sars slipsite to U UUA AAU AmpR Ura3 dual luciferase 9274 2u ColE1 Genbank_Plasmid_Maps/pJD662.gb Site directed mutagenesis of pJD465 to make the slippery sites U UUA AAU
pJD0679 pJD465 sars slipsite to U UUA AAG AmpR Ura3 dual luciferase 9274 2u ColE1 Genbank_Plasmid_Maps/pJD662.gb Site directed mutagenesis of pJD465 to make the slippery sites U UUA AAG
pJD0680 pJD465 sars slipsite to U UUA AAA AmpR Ura3 dual luciferase 9274 2u ColE1 Genbank_Plasmid_Maps/pJD662.gb Site directed mutagenesis of pJD465 to make the slippery sites U UUA AAA
pJD0681 pJD502 sars slipsite to U UUA AAU AmpR
dual luciferase 5480
ColE1 Genbank_Plasmid_Maps/pJD662.gb Site directed mutagenesis of pJD502 to make the slippery sites U UUA AAU
pJD0682 pJD502 sars slipsite to U UUA AAG AmpR
dual luciferase 5480
ColE1 Genbank_Plasmid_Maps/pJD662.gb Site directed mutagenesis of pJD502 to make the slippery sites U UUA AAG
pJD0683 pJD502 sars slipsite to U UUA AAA AmpR
dual luciferase 5480
ColE1 Genbank_Plasmid_Maps/pJD662.gb Site directed mutagenesis of pJD502 to make the slippery sites U UUA AAA
pJD0684 pJD375 Zero frame Renilla delta AmpR Ura3 dual luciferase 8478
ColE1 Genbank_Plasmid_Maps/pJD684.gb SDM of pJD375 to make a sal 1 site, then cut it and religate to produce pJD375 Renilla delta.
pJD0685 pJD641 Mutate EST2 slipsite position 1352 AmpR Leu2 est2 9738 Cen ColE1 Genbank_Plasmid_Maps/pJD685.gb SDM of pJD641 to change the 1325 slipsite from ccA AAA AAA to gaA AAT TTA AAA
pJD0686 pJD641 Mutate EST2 slipsite position 72 AmpR Leu2 est2 9738 Cen ColE1 Genbank_Plasmid_Maps/pJD686.gb SDM of pJD641 to change the 1325 slipsite from ccA AAA AAA to gaG AAC TTG AAG
pJD0687 pRS426
AmpR Ura3
5374 2u ROP Genbank_Plasmid_Maps/pJD741.gb Yeast vector based on pRS426 (E. coli low copy number). Restriction sites at MCS are labeled
pJD0688 pJD426 TET cassette (TetO 2 repeats) AmpR Ura3
7966 2u ROP Genbank_Plasmid_Maps/pJD741.gb TET cassette (TetO 2 repeats) from pJD426 subcloned at HindIII/EcoRI sites
pJD0689 pJD427 TET cassette (TetO 7 repeats) AmpR Ura3
8184 2u ROP Genbank_Plasmid_Maps/pJD741.gb TET cassette (TetO 7 repeats) from pJD427 subcloned at HindIII/EcoRI sites
pJD0690 pJD688 RDN5 AmpR Ura3
8491 2u ROP Genbank_Plasmid_Maps/pJD741.gb PCR fragment containing RDN5 cloned to pJD688 at HindIII site
pJD0691 pJD689 RDN5 AmpR Ura3
8709 2u ROP Genbank_Plasmid_Maps/pJD741.gb PCR fragment containing RDN5 cloned to pJD689 at HindIII site
pJD0692 pJD690 RDN35 AmpR Ura3
15404 2u ROP Genbank_Plasmid_Maps/pJD741.gb PCR fragment containing RDN35 fragment cloned to pJD690 at a NotI/BamHI site
pJD0693 pJD691 RDN35 AmpR Ura3
15622 2u ROP Genbank_Plasmid_Maps/pJD741.gb PCR fragment containing RDN35 cloned to pDJ691 at NotI/BamHI site
pJD0694 pJD692 HygR into RDN18 AmpR Ura3
15404 2u ROP Genbank_Plasmid_Maps/pJD741.gb HygR introduced by SDM into pJD692
pJD0695 pJD693 HygR into RDN18 AmpR Ura3
15622 2u ROP Genbank_Plasmid_Maps/pJD741.gb HygR introduced by SDM into pJD693
pJD0696 pJD656 C CCA AAC AmpR Ura3
9274 2u
Genbank_Plasmid_Maps/pJD741.gb Different SARS slippery sites: C CCA AAC subcloned from pJD656
pJD0697 pJD657 A AAA AAC AmpR Ura3
9274 2u
Genbank_Plasmid_Maps/pJD741.gb Different SARS slippery sites: A AAA AAC subcloned from pJD657
pJD0698 pJD658 G GGU UUU AmpR Ura3
9274 2u
Genbank_Plasmid_Maps/pJD741.gb Different SARS slippery sites: G GGU UUU subcloned from pJD658
pJD0699 pJD642 missense





Genbank_Plasmid_Maps/pJD741.gb Jen, missense
pJD0700 pJD643 missense





Genbank_Plasmid_Maps/pJD741.gb Jen, missense
pJD0701 pJD644 missense





Genbank_Plasmid_Maps/pJD741.gb Jen, missense
pJD0702 431 missense





Genbank_Plasmid_Maps/pJD741.gb Jen, missense
pJD0703 432 432 in 408 (leu2)





Genbank_Plasmid_Maps/pJD741.gb Jen, missense, 432 in 408 (leu2)
pJD0704 433 missense





Genbank_Plasmid_Maps/pJD741.gb Jen, missense, 433
pJD0705
tet DL plasmid, 0 frame





Genbank_Plasmid_Maps/pJD741.gb TET DL plasmids 0 frame
pJD0706
tet DL plasmid LA





Genbank_Plasmid_Maps/pJD741.gb TET DL plasmid LA
pJD0707
tet DL plasmid Ty1





Genbank_Plasmid_Maps/pJD741.gb TET DL plasmid Ty1
pJD0708
tet DL plasmid HIV





Genbank_Plasmid_Maps/pJD741.gb TET DL plasmid HIV
pJD0709
tet DL plasmid UAA





Genbank_Plasmid_Maps/pJD741.gb TET DL plasmid UAA
pJD0710
tet DL plasmid PTC





Genbank_Plasmid_Maps/pJD741.gb TET DL plasmid PTC
pJD0711
tet DL plasmid LA FS to stop





Genbank_Plasmid_Maps/pJD741.gb tet DL plasmid LA fs to stop
pJD0712
tet DL plasmid 0 frame LA (LA FS out of frame)





Genbank_Plasmid_Maps/pJD741.gb tet DL plasmid 0 frame LA (LA FS out of frame)
pJD0713







Genbank_Plasmid_Maps/pJD741.gb 0.1%
pJD0714







Genbank_Plasmid_Maps/pJD741.gb 0.5%
pJD0715







Genbank_Plasmid_Maps/pJD741.gb 3.5%
pJD0716







Genbank_Plasmid_Maps/pJD741.gb 5%
pJD0717







Genbank_Plasmid_Maps/pJD741.gb 12%
pJD0718







Genbank_Plasmid_Maps/pJD741.gb FS to stop
pJD0719







Genbank_Plasmid_Maps/pJD741.gb FS to stop
pJD0720







Genbank_Plasmid_Maps/pJD741.gb FS to stop
pJD0721







Genbank_Plasmid_Maps/pJD741.gb FS to stop
pJD0722







Genbank_Plasmid_Maps/pJD741.gb FS to stop
pJD0723
15Kd





Genbank_Plasmid_Maps/pJD741.gb Haichen 15Kd
pJD0724
12Kd





Genbank_Plasmid_Maps/pJD741.gb Haichen 12Kd
pJD0725
12Kd A





Genbank_Plasmid_Maps/pJD741.gb Haichen 12Kd, A
pJD0726
12Kd G





Genbank_Plasmid_Maps/pJD741.gb Haichen 12Kd, G after stop
pJD0727
12Kd T





Genbank_Plasmid_Maps/pJD741.gb Haichen 12Kd, T
pJD0728
pCMV-Gl





Genbank_Plasmid_Maps/pJD741.gb Maquat, pCMV-Gl
pJD0729
pCMV-GPx1





Genbank_Plasmid_Maps/pJD741.gb Maquat, pCMV-GPx1
pJD0730 pKT209

Ura3



Genbank_Plasmid_Maps/pJD741.gb pFA6a-link-yEGFP-CaURA3
pJD0731 pKT210

HIS5



Genbank_Plasmid_Maps/pJD741.gb pFA6a-link-yEmCFP-5pHIS5 / yECFPA206R
pJD0732 pKT211

HIS5



Genbank_Plasmid_Maps/pJD741.gb pFA6a-link-yEmCitrine-5pHIS5 / yECitrineA206R
pJD0733 pKT212

Ura3



Genbank_Plasmid_Maps/pJD741.gb pFA6a-link-yEmCFP-CaURA3 / yECFP A206R
pJD0734 pKT220
KanR




Genbank_Plasmid_Maps/pJD741.gb pFAba-link-yECitrine-3HA-KanR / yECitrine-3HA
pJD0735 pKT221
KanR




Genbank_Plasmid_Maps/pJD741.gb pFAba-link-yECFP-3HA-KanR / yECFP-3HA
pJD0736 pKT232
KanR




Genbank_Plasmid_Maps/pJD741.gb pFAba-link-yECFP-13Myc-KanR
pJD0737 pKT233

His5



Genbank_Plasmid_Maps/pJD741.gb pFAba-link-yECitrine-13Myc-KanR
pJD0738 pKT239

His5



Genbank_Plasmid_Maps/pJD741.gb pFAba-link-yECitrine-3HA-5pHis5
pJD0739 pKT240

His5



Genbank_Plasmid_Maps/pJD741.gb pFAba-link-yECitrine-13Myc-5pHis5
pJD0741

AmpR Ura PGK1
Cen6
Genbank_Plasmid_Maps/pJD741.gb

pJD0742 pJD375 ppr1.660 AmpR Ura dual lux
Cen
Genbank_Plasmid_Maps/pJD742.gb

pJD0743 pJD375 ppr1.1188 AmpR Ura dual lux
Cen
Genbank_Plasmid_Maps/pJD743.gb

pJD0744 pJD375 ppr1.1260 AmpR Ura dual lux
Cen
Genbank_Plasmid_Maps/pJD744.gb

pJD0745 pJD375 ppr1.2094 AmpR Ura dual lux
Cen
Genbank_Plasmid_Maps/pJD766.gb

pJD0746 pJD375 ppr1.525 AmpR Ura dual lux
Cen
Genbank_Plasmid_Maps/pJD746.gb

pJD0747 pJD741 bub3 backwards AmpR Ura PGK1
Cen6
Genbank_Plasmid_Maps/pJD747.gb

pJD0748 pJD741 SPR6 3196-3393 AmpR Ura PGK1
Cen6
Genbank_Plasmid_Maps/pJD748-spr6.gb

pJD0753 pJD741 Renilla/Ffly 375 fragment AmpR Ura PGK1
Cen6
Genbank_Plasmid_Maps/pJD753.gb

pJD0754 pJD741 (pJD662)EST2 1611-1800 + pJD375 AmpR Ura PGK1
Cen6
Genbank_Plasmid_Maps/pJD754.gb

pJD0755 pJD741 (pJD668)BUB3 795-939 + pJD375 AmpR Ura PGK1
Cen6
Genbank_Plasmid_Maps/pJD755.gb

pJD0756 pJD741 (pJD670)TBF1 1488-1686 + pJD375 AmpR Ura PGK1
Cen6
Genbank_Plasmid_Maps/pJD756.gb

pJD0757 pJD741 (pJD671)CTS2 1155-1359 + pJD375 AmpR Ura PGK1
Cen6
Genbank_Plasmid_Maps/pJD757.gb

pJD0758 pJD741 ppr1.660 (pJd742) AmpR Ura PGK1
Cen6
Genbank_Plasmid_Maps/pJD758.gb

pJD0759 pJD741 ppr1.1188 (pJD743) AmpR Ura PGK1
Cen6
Genbank_Plasmid_Maps/pJD759.gb

pJD0760 pJD741 ppr1.1260 (pJD744) AmpR Ura PGK1
Cen6
Genbank_Plasmid_Maps/pJD760.gb

pJD0761 pJD741 ppr1.2094 (pJD745) AmpR Ura PGK1
Cen6
Genbank_Plasmid_Maps/pJD761.gb

pJD0765 pJD741 proper PTC AmpR
PGK1
Cen6
Genbank_Plasmid_Maps/pJD766.gb

pJD0766 pJD741 (pJD660)EST2 1174-1305 + pJD375





Genbank_Plasmid_Maps/pJD766.gb

pJD0768 pJD175e MHV AmpR




Genbank_Plasmid_Maps/pJD766.gb The MHV frameshift signal was obtained by PCR, cloned as a BamHI/SacI fragment into pJD175e. This contains 50nt upstream from the slippery site.
pJD0769 pJD175f / pJD768 MHV AmpR




Genbank_Plasmid_Maps/pJD766.gb pJD769 is the BamHI/EcoRI fragment from pJD768 cloned into pJD175f
pJD0770 ?






Genbank_Plasmid_Maps/pJD766.gb

pJD0771 ?






Genbank_Plasmid_Maps/pJD766.gb

pJD0772 pJD768 MHV AmpR




Genbank_Plasmid_Maps/pJD766.gb Delta S2
pJD0773 pJD769 MHV AmpR




Genbank_Plasmid_Maps/pJD766.gb Delta S2
pJD0774 pJD768 MHV AmpR




Genbank_Plasmid_Maps/pJD766.gb Delta L3
pJD0775 pJD769 MHV AmpR




Genbank_Plasmid_Maps/pJD766.gb Delta L3
pJD0776 pJD768 MHV AmpR




Genbank_Plasmid_Maps/pJD766.gb Delta S2, Delta L3
pJD0777 pJD769 MHV AmpR




Genbank_Plasmid_Maps/pJD766.gb Delta S2, Delta L3
pJD0778 pJD768 MHV AmpR




Genbank_Plasmid_Maps/pJD766.gb S2b, Delta L3
pJD0779 pJD769 MHV AmpR




Genbank_Plasmid_Maps/pJD766.gb S2b, Delta L3
pJD0780 pJD768 MHV AmpR




Genbank_Plasmid_Maps/pJD766.gb Delta S2, Delta L3, S2b
pJD0781 pJD769 MHV AmpR

5559

Genbank_Plasmid_Maps/pJD766.gb Delta S2, Delta L3, S2b
pJD0782 pJD768 MHV AmpR




Genbank_Plasmid_Maps/pJD766.gb Delta S2, S2b, Delta L3, S3b
pJD0783 pJD769 MHV AmpR




Genbank_Plasmid_Maps/pJD766.gb Delta S2, S2b, Delta L3, S3b
pJD0784 pJD768 MHV AmpR




Genbank_Plasmid_Maps/pJD766.gb S2b, Delta L3, S3b
pJD0785 pJD769 MHV AmpR




Genbank_Plasmid_Maps/pJD766.gb S2b, Delta L3, S3b
pJD0786 pBluescript (pJD175c?) HIV AmpR
Dual Luciferase 5854
ColE1 Genbank_Plasmid_Maps/pJD766.gb Dual Luciferase HIV frameshift plasmids from Atkins/Gesteland group
pJD0787 pBluescript/pJD175d HIV AmpR
Dual Luciferase 5855
ColE1 Genbank_Plasmid_Maps/pJD766.gb Dual Luciferase HIV frameshift plasmids from Atkins/Gesteland group, slippery heptamer mutants and one mutant downstream (n->T)
pJD0788 pJD526 / pBluescript SARS AmpR

5926
ColE1 Genbank_Plasmid_Maps/pJD766.gb Plasmid for transcribing SARS control RNA, BamHI/SacI from pJD526 cloned into pBluescript (polyU instead of UUUAAAC), +strand RNA can be made from T7, -strand from T3.
pJD0789 pJD502
AmpR




Genbank_Plasmid_Maps/pJD789.gb Disrupt structure, mutations in pJD502 stocks made by Ewan 4/4/6
pJD0790 pJD502
AmpR




Genbank_Plasmid_Maps/pJD790.gb "Assymetrical loop?"
pJD0791 pJD502
AmpR




Genbank_Plasmid_Maps/pJD791.gb Conserve Structure
pJD0792 pJD502
AmpR




Genbank_Plasmid_Maps/pJD792.gb Disrupt Structure
pJD0793 pJD507
AmpR




Genbank_Plasmid_Maps/pJD793.gb Assymetrical loop
pJD0794 pJD507
AmpR




Genbank_Plasmid_Maps/pJD794.gb Conserve Structure
pJD0795 pJd507
AmpR




Genbank_Plasmid_Maps/pJD795.gb Disrupt Structure
pJD0796 pJD641
AmpR Leu2 EST2 9738 Cen ColE1 Genbank_Plasmid_Maps/pJD796.gb This is a leu based shuttle vector with the EST2 CDS sequence following LacO/M13/MCS, the slippery sites at positions 72,1215,1326,1653, and 1995 have been disrupted via SDM
pJD0797 pJD520
AmpR Ura3 Dual luciferase 9286 Cen ColE1 Genbank_Plasmid_Maps/pJD797.gb This is a mutation of pJD520 with the 7 bases comprising stem 1 mutated to their complements.
pJD0798







Genbank_Plasmid_Maps/pJD798.gb Terry Kinzy, TKB900, pDB0868: CTY 775/Luc CAAA FF H245 CAC to CGC
pJD0799







Genbank_Plasmid_Maps/pJD799.gb Terry Kinzy, TKB901, pDB0691:CTY775/Luc UGAC
pJD0800







Genbank_Plasmid_Maps/pJD800.gb Terry Kinzy, TKB902, pDB0690:CTY775/Luc CGAC
pJD0801







Genbank_Plasmid_Maps/pJD801.gb Terry Kinzy, TKB903, pDB00688: CTY775/Luc CAAA
pJD0802 pRS316






Genbank_Plasmid_Maps/pJD802.gb p1052, GCN2-M788V,E1606G
pJD0803 pRS316






Genbank_Plasmid_Maps/pJD803.gb p1053, GCN2-R768W,D987G,E1606G
pJD0804 pRS316






Genbank_Plasmid_Maps/pJD804.gb p1054, GCN2-E601K, E1606G
pJD0805 pRS316






Genbank_Plasmid_Maps/pJD805.gb p1056, GCN2-E601K, E1591K
pJD0806 pJD754 wobble bases of EST2 slipsite mutagenized AmpR Ura3 PGK1 7325 2u ColE1 Genbank_Plasmid_Maps/pJD806.gb The parent of this ispJD754, URA PGK1 vector with genomic EST2 PRF signal between small portion of renilla/firefly inside PGK1. The wobble bases of the slippery site have been mutagenized.
pJD0807 pJD757 CTS2 wobble bases mutagenized AmpR Ura3 PGK1 7340 Cen6 ColE1 Genbank_Plasmid_Maps/pJD807.gb The parent of this vector is pJD757. It contains the CTS2 PRF signal inside the PGK1 ORF, the wobble bases of the slippery site have been mutagenized.
pJD0808 pJD754 EST2 wobble bases mutagenized AmpR Ura3 dual luciferase 9283 Cen6 ColE1 Genbank_Plasmid_Maps/pJD808.gb The EST2 PRF signal between firefly and renilla, the slipsite has been changed from A AAA AAT to G AAG AAC
pJD0809

AmpR Ura3
15472 2u ColE1 Genbank_Plasmid_Maps/pJD1120.gb pTET AptH59, aptamer
pJD0810 MR6 L11 in pRS314 TRP AmpR Trp



Genbank_Plasmid_Maps/pJD1120.gb

pJD0811 pJD754 EST2 stem1 disruption AmpR Ura3 PGK1 7325 Cen6 ColE1 Genbank_Plasmid_Maps/pJD1120.gb This is a PGK1 stability vector with EST2 and stem1 complementations.
pJD0812 pJD755 BUB3 slippery site disruption AmpR Ura3 PGK1 7280 Cen6 ColE1 Genbank_Plasmid_Maps/pJD1120.gb This is a PGK1 stability vector with BUB3 slippery site disruptions
pJD0813 pJD756 TBF1 slippery site disruption AmpR Ura3 PGK1 7334 Cen6 ColE1 Genbank_Plasmid_Maps/pJD1120.gb This is a PGK1 stability vector with TBF1 slippery site disruptions
pJD0814 pJD756 TBF1 stem 1 disruption AmpR Ura3 PGK1 7334 Cen6 ColE1 Genbank_Plasmid_Maps/pJD1120.gb This is a PGK1 stability vector with TBF1 stem 1 disruptions
pJD0815 pJD757 CTS2 stem 1 disruption AmpR Ura3 PGK1 7340 Cen6 ColE1 Genbank_Plasmid_Maps/pJD1120.gb This is a PGK1 stability vector with CTS2 slippery site disruptions
pJD0816 pJD759 PPR1 slippery site disruption AmpR Ura3 PGK1 7325 Cen6 ColE1 Genbank_Plasmid_Maps/pJD1120.gb This is a PGK1 stability vector with PPR1 slippery site disruptions
pJD0817 pJD748 SPR6 stem1 disruption AmpR Ura3 PGK1 7246 Cen6 ColE1 Genbank_Plasmid_Maps/pJD1120.gb This is a PGK1 stability vector with SPR6 stem 1 disruptions
pJD0818 pJD748 SPR6 slippery site disruption AmpR Ura3 PGK1 7246 Cen6 ColE1 Genbank_Plasmid_Maps/pJD1120.gb This is a PGK1 stability vector with SPR6 slippery site disruptions
pJD0819 pCMV6-XL4 CCR5 cDNA AmpR

5766
ColE1 Genbank_Plasmid_Maps/pJD1120.gb This vector did not work well for cloning out CCR5, we also have pJD174
pJD0820 pJD376 A before LA frameshift signal to make 0 frame control AmpR Ura3 dual luciferase
Cen6 ColE1 Genbank_Plasmid_Maps/pJD1120.gb DL plasmid: pJD376 mutated to a 0 frame control by adding an A before the LA FS signal.
pJD0821 p180 GCN4-lacZ
Ura3



Genbank_Plasmid_Maps/pJD1120.gb

pJD0822 p226 GCN4-lacZ
Ura3



Genbank_Plasmid_Maps/pJD1120.gb

pJD0823 p227 GCN4-lacZ
Ura3



Genbank_Plasmid_Maps/pJD1120.gb

pJD0824 pM199 GCN4-lacZ
Ura3



Genbank_Plasmid_Maps/pJD1120.gb

pJD0825 pM226 GCN4-lacZ
Ura3



Genbank_Plasmid_Maps/pJD1120.gb

pJD0826 pG30 GCN4-lacZ
Ura3



Genbank_Plasmid_Maps/pJD1120.gb

pJD0827 pJD175e / pJD819 CCR5 AmpR
dual luciferase 5624
ColE1 Genbank_Plasmid_Maps/pJD827.gb This dual luciferase vector contains 226 bases of homo sapiens CCR5 between Renilla and Firefly PCR amplified from pJD819
pJD0828 pJD753 premature termination codon AmpR Ura3 PGK1 7164 Cen6 ColE1 Genbank_Plasmid_Maps/pJD828.gb Created from pJD753 by cutting with AVR2 and backfilling using the Klenow fragment. Stop codon introduced 555 bases into the PGK1 ORF
pJD0829 pAJ1973 pRS426-GPD-GST-U1Ap





Genbank_Plasmid_Maps/pJD829.gb

pJD0830 pAJ1181 rDNA with U1A site inserted


14912

Genbank_Plasmid_Maps/pJD830.gb rRNA with U1A binding site + PST1 site in pAJ718
pJD0831
CCA adding enzyme AmpR




Genbank_Plasmid_Maps/pJD831.gb tRNA terminal nucleotidyl transferase, CCA adding enzyme, HIS tagged
pJD0832
CMRS, E.coli methionyl tRNA synthetase AmpR




Genbank_Plasmid_Maps/pJD832.gb E. coli methionyl tRNA synthetase
pJD0833
YFRS, phenylalanine tRNA synthetase AmpR




Genbank_Plasmid_Maps/pJD662.gb yeast phenylalanine tRNA synthetase
pJD0834 pDB698 BL21 RF1 clone 6xHis





Genbank_Plasmid_Maps/pJD834.gb RF1 clone 6xHis from David Bedwell, pDB698 BL21 (DE3)
pJD0835 pJD175e homo sapiens interleukin 7 receptor alpha AmpR
dual luciferase 5531
ColE1 Genbank_Plasmid_Maps/pJD835.gb pJD175e containing sequence from BC067540 (homo sapiens ile7r) bases 948 to 1161. Three overlapping potential slippery sites (ccaagAAAAAATTTTAAT) The t between the two lowercase ts has been removed to negate -1 frame stop codons.
pJD0836 pJD175e mouse interleukin 7 frameshift signal AmpR none dual luciferase 5549 none ColE1/ Genbank_Plasmid_Maps/pJD836.gb A U was removed immediately after the frameshift signal Sharmishtha
pJD0837 pJD502 mutagenized S3C AmpR
Dual luciferase

ColE1 Genbank_Plasmid_Maps/pJD837.gb SARS Mutants made by Jess, S3C
pJD0838 pJD473 mutagenized S23C AmpR
Dual luciferase


Genbank_Plasmid_Maps/pJD838.gb SARS Mutants made by Jess, S23C
pJD0839 pJD502 L2* AmpR
Dual luciferase


Genbank_Plasmid_Maps/pJD839.gb SARS Mutants made by Jess, L2*
pJD0840 pJD473 S2C* AmpR
Dual luciferase


Genbank_Plasmid_Maps/pJD840.gb SARS Mutants made by Jess, S2C*
pJD0841 pJD473 S3_2bp_CUUG AmpR
Dual luciferase


Genbank_Plasmid_Maps/pJD841.gb SARS Mutants made by Jess, S3_2bp_CUUG
pJD0842 pJD507/pJD175e SARS frameshift signal and attenuator AmpR
Dual luciferase 5630
ColE1 Genbank_Plasmid_Maps/pJD842.gb SARS frameshift signal and attenuator region; amplified from pJD507, cut with SacI and SalI, cloned into pJD175e
pJD0843 pJD507/pJD175e SARS frameshift signal and attenuator, 0 frame control AmpR
Dual luciferase

ColE1 Genbank_Plasmid_Maps/pJD843.gb SARS frameshift signal and attenuator region; amplified from pJD507, cut with SacI and SalI, cloned into pJD175e, 0 frame control
pJD0844 pJD827 CCR5 slipsite mutant AmpR
Dual luciferase 5624
ColE1 Genbank_Plasmid_Maps/pJD844.gb CCR5 mutants made by Jess, SDM from pJD827, slip site mutant
pJD0845 pJD827 CCR5 stem2 5' side AmpR
Dual luciferase 5624
ColE1 Genbank_Plasmid_Maps/pJD845.gb CCR5 mutants made by Jess, SDM from pJD827, stem 2 5' side
pJD0846 pJD827 CCR5 stem2 3' side AmpR
Dual luciferase 5624
ColE1 Genbank_Plasmid_Maps/pJD846.gb CCR5 mutants made by Jess, SDM from pJD827, stem 2 3' side
pJD0847 pJD175e homo sapiens interleukin 27 receptor AmpR
Dual luciferase 5540
ColE1 Genbank_Plasmid_Maps/pJD847.gb This plasmid contains the homo sapiens interlekin receptor 27 PRF signal between firefly and renilla. This was cloned using the oligo PCR method.
pJD0848 pJD175e CCR5 stem 2 mutant (both sides complementation) Amp none dual luciferase 5625
ColE1 Genbank_Plasmid_Maps/pJD848.gb CCR5 in 175e with stem 2 compensatory mutations trey, bryan
pJD0849 pTET-Off none AmpR none
7391
ColE1 Genbank_Plasmid_Maps/pJD849.gb
Brewer lab
pJD0850 pJD175e CCR5 in 175e AmpR

5624

Genbank_Plasmid_Maps/pJD850.gb

pJD0851 pJD175e CCR5 complementary mutations in 5' of stem 1 AmpR

5624

Genbank_Plasmid_Maps/pJD851.gb

pJD0852 pJD175e CCR5 AmpR none dual luciferase 5624
ColE1/ Genbank_Plasmid_Maps/pJD852.gb

pJD0853 p406 Reed Wickner Ura3 disruption clone of MAK10





Genbank_Plasmid_Maps/pJD853.gb

pJD0854 pJD175e CCR5 PRF AmpR none dual luciferase 5624
ColE1/ Genbank_Plasmid_Maps/pJD854.gb 9 bases of CCR5 PRF stem 1 mutated to the corresponding bases of the 3' end
pJD0855 pJD175e CCR5 PRF AmpR none dual luciferase 5624
ColE1/ Genbank_Plasmid_Maps/pJD855.gb Both sides of potential CCR5 PRF stem 1 have been mutated to the corresponding bases of the other side.
pJD0856 pJD175e CCR5 PRF AmpR none dual luciferase 5624
ColE1/ Genbank_Plasmid_Maps/pJD856.gb 5' portion of the proximal region of the computationally identified stem 2 is changed.
pJD0857 pJD175e CCR5 PRF AmpR none dual luciferase 5624
ColE1/ Genbank_Plasmid_Maps/pJD857.gb 3' portion of the computationally identified region of stem 2 is mutated
pJD0858 pJD175e CCR5 PRF AmpR none dual luciferase 5624
ColE1/ Genbank_Plasmid_Maps/pJD858.gb Both sides of stem 2 of the CCR5 PRF signal have been modified to make a compensatory stem.
pJD0859 pJD375-pdr5-300 PDR5 PRF AmpR URA3 dual luciferase 9328 ColE1/CEN6 ColE1/CEN6 Genbank_Plasmid_Maps/pJD859.gb PDR5 PRF is ~ 300 bp into the gene.
pJD0860 pJD375-pdr5-3027 PDR5 PRF AmpR URA3 dual luciferase 9328 ColE1/CEN6 ColE1/CEN6 Genbank_Plasmid_Maps/pJD860.gb PDR5 PRF is ~3027 bp into the gene, but did not promote frame-shifting very well.
pJD0861 pJD180 5S rDNA AmpR TRP1

/2u
Genbank_Plasmid_Maps/pJD861.gb U2860A
pJD0862 pJD180 5S rDNA AmpR TRP1

/2u
Genbank_Plasmid_Maps/pJD862.gb U2860C
pJD0863 pJD180 5S rDNA AmpR TRP1

/2u
Genbank_Plasmid_Maps/pJD863.gb U2860G
pJD0864 pJD180 5S rDNA AmpR TRP1

/2u
Genbank_Plasmid_Maps/pJD863.gb A2940C
pJD0865 pJD180 5S rDNA AmpR TRP1

/2u
Genbank_Plasmid_Maps/pJD863.gb A2940G
pJD0866 pJD180 5S rDNA AmpR TRP1

/2u
Genbank_Plasmid_Maps/pJD863.gb A2940U
pJD0867 pJD180 5S rDNA AmpR TRP1

/2u
Genbank_Plasmid_Maps/pJD867.gb C2941U
pJD0868 pJD398 XylA (S. degradans) AmpR TRP1

CEN
Genbank_Plasmid_Maps/pJD863.gb

pJD0869 pJD399 XylA (S. degradans) AmpR TRP1

2mkn
Genbank_Plasmid_Maps/pJD863.gb

pJD0870 pJD402 XylB (S. degradans) AmpR URA3

CEN
Genbank_Plasmid_Maps/pJD863.gb

pJD0871 pJD403 XylB (S. degradans) AmpR URA3

2mkn
Genbank_Plasmid_Maps/pJD863.gb

pJD0872 pJD409 XKS1 (S. cerevisiae) AmpR URA3

CEN
Genbank_Plasmid_Maps/pJD863.gb

pJD0873 pJD396 TAL1 (S. cerevisiae) AmpR HIS3

CEN
Genbank_Plasmid_Maps/pJD863.gb

pJD0874 pJD402 TKL1 (S. cerevisiae) AmpR URA3

CEN
Genbank_Plasmid_Maps/pJD863.gb

pJD0875 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb G1019A
pJD0876 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb G1019C
pJD0877 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb G1019U
pJD0878 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb G1020A
pJD0879 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb G1020C
pJD0880 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb G1020U
pJD0881 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb G1021A
pJD0882 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb G1021C
pJD0883 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb G1021U
pJD0884 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb AAA[B1a]CCC
pJD0885 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb A1025C
pJD0886 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb A1025G
pJD0887 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb A1025U
pJD0888 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb A1026C
pJD0889 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb A1026G
pJD0890 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb A1026U
pJD0891 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb A1027C
pJD0892 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb A1027G
pJD0893 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb A1027U
pJD0894 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb U2860A
pJD0895 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb U2860C
pJD0896 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb U2860G
pJD0897 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb U2860D
pJD0898 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb C2924A
pJD0899 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb C2924G
pJD0900 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb C2924U
pJD0901 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb C2924D
pJD0902 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb A2940C
pJD0903 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb A2940G
pJD0904 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb A2940U
pJD0905 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb A2940D
pJD0906 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb C2941A
pJD0907 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb C2941G
pJD0908 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb C2941U
pJD0909 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb C2941D
pJD0910 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb U2948A
pJD0911 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb U2948C
pJD0912 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb U2948G
pJD0913 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb U2948D
pJD0914 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb G2621A
pJD0915 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb G2621C
pJD0916 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb G2621U
pJD0917 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb G2621D
pJD0918 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb G2814A
pJD0919 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb G2814U
pJD0920 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb A2818U
pJD0921 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb A2818G
pJD0922 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb A2818C
pJD0923 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb A2819U
pJD0924 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb A2819G
pJD0925 pTET Mutant 25S rRNA AmpR URA3

/2u
Genbank_Plasmid_Maps/pJD863.gb A2819C
pJD0926 pTET Mutant 25S rRNA AmpR URA3

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